European Cytoskeletal Forum
European Cytoskeletal Forum Meeting 2022
Scientific programme at a glance:
Monday 16 May 2022
Minimal systems, reconstituted systems (Chair: Jan Faix / Cornelia Lee-Thedieck)
09.00 – 09:05 Welcome address
09.05 – 10:05 Actin and microtubules, the enemy brothers
Manuel Thery, Hopital Saint Louis, Paris / Laurent Blanchoin, iRTSV Grenoble
10.05 – 10.35 Cytoskeletal crosstalk: when four different personalities team up
Gijsje H. Koenderink, TU Delft
10.35 – 11.05 Pattern formation in active cytoskeletal and cellular systems
Andreas Bausch, TU Munich
11.05 – 11.30 Coffee Break
11.30 – 12.30 Selected talks from abstracts
[T1] Stability of branched versus linear Arp2/3-generated actin filaments
Luyan Cao (YIA), Francis Crick Institute
[T2] Transient presence of exploratory microtubules initiates formation of prevailing actin bundles through CKAP5-dependent cross-linking
Ján Sabó (YIA) Vestec/Prague
[T3] Multicomponent mechanisms of actin depolymerization - the forgotten child of actin dynamics Shashank Shekhar, EMORY University
[T4] Evolution and actin isoforms: Insights on actin network segregation
Micaela Boiero Sanders, Dortmund/Marseille
12.30 – 14.00 Lunch
Cytoskeletal structure (Chair: Kay Grünewald, Germany / Stefan Raunser)
14.00 – 14:30 Allosteric modulation of force production of myosin motors
Anne Houdusse, Institut Curie
14.30 – 15.00 Cryo-ET reveals sarcomere structures at molecular resolution
Stefan Raunser, MPI Dortmund
15.00 – 15.30 Molecular Mechanisms of Arp2/3 Complex Activation and Inhibition
Roberto Dominguez, University of Pennsylvania
15.30 – 16.00 Coffee Break
16.00 – 16.35 Selected talks from abstracts
16.00 – 16.05 3i Technobite Presentation:
Advanced Multimodal Microscope Systems for Cytoskeleton Research
[T29] The end is the beginning - How capping protein promotes nucleation in branched actin actin networks
Peter Bieling, MPI Dortmund
[T30] WASH complex: membrane attachment revised
Lenka Libusová, Charles University Prague
16.00 – 19.15 Poster Session I: P1 – P33
19.30 – 21.30 Dinner @ Altes Rathaus
Tuesday 17 May 2022
Cytoskeleton and Disease (Chair: Tim Gilberger / Stefan Linder)
09.00 – 09.30 Cytoskeletal proteins and disease
Michelle Peckham, University of Leeds
09.30 – 10.00 Structure, Regulation, and Mechanisms of Myosin-2 Motors
Sarah Heissler, The Ohio State University
10.00 – 10.30 Chemical targeting of tropomyosins: From biology to drug development
Peter Gunning, UNSW Sydney
10.30 – 11.00 Unusual dynamics and functions of microtubules and actin filaments in Plasmodium
Friedrich Frischknecht, Universität Heidelberg
11.00 – 11.25 Coffee Break
11.25 – 11.55 Illuminating the beginning of life
Melina Schuh, MPI Göttingen
12.00 – 12:40 Selected talks from abstracts
[T9] Genetic and mechanistic dissection of non-muscle actinopathies caused by ACTB or ACTG1 variants
Nataliya DiDonato, TU Dresden – Carl Gustav Carus University Hospital
[T10] Biochemical and mechanical characterization of beta-actin mutants causing non-muscle actinopathies
Irene Pertici (YIA), University of Florence
[T11] Non-muscle actin paralogues differ in their binding properties to the Arp2/3 complex influencing F-actin organization in human melanoma cells
Antonina Mazur, University of Wroclaw
12.40 – 14.00 Lunch
Migration and Invasion (Chair: Beate Sodeik / Philippe Chavrier)
14.00 – 14.30 The protease-dependent program of breast tumor invasion
Philippe Chavrier, Institut Curie
14.30 – 15.00 Non-muscle myosin II expression and regulation in the control of immune function
Miguel Vincente Manzanares, iBMCC Salamanca
15.00 – 15.30 Unexpected roles of actin in nuclear envelope breakdown
Peter Lénárt, MPI Göttingen
15.30 – 16.00 Coffee Break
16.00 – 16.30 Chemotaxis in real physiology – self-generated gradients are everywhere
Robert H. Insall, Beatson Institute Glasgow
16.30 – 17.15 Selected talks from abstracts
[T12] Identification of a new actin polymerization function of the WRC independent of Arp2/3, but dependent on its polyproline domains
Simona Buracco (YIA), Beatson Institute for Cancer Research
[T13] Calcium bursts allow rapid reorganization of EFhD2/Swip-1 crosslinked actin networks in epithelial wound closure
Franziska Lehne (YIA), University of Marburg
[T14] Directed cell invasion and differential adhesion drive tissue elongation and turning in C. elegans gonad morphogenesis
Ronen Zaidel-Bar, Tel Aviv University
19.30 – 22.30 Conference Dinner Banquet at Hotel Courtyard Hannover Maschsee
Wednesday 18 May 2022
Cell Shape and Division (Chair: Miguel Vicente-Manzanares / Sarah Heissler)
09.00 – 9.30 Cellular morphogenesis across scales: from molecules to forces
Ewa K. Paluch, University of Cambridge
09.30 – 10:00 Cytoskeletal Mechanisms of Axon Growth and Regeneration
Frank Bradke, DZNE Bonn
10.00 – 10.45 Selected talks from abstracts
[T15] Nanoscale organization of the midbody
Nadja Hümpfer, FU Berlin
[T16] Actin cortex mechanics probed in live cells
Joseph Vermeil, Institut Curie Paris
[T17] CAP1 and cofilin1: an intimate duet that governs neuronal actin dynamics
Marco Rust, Philipps-University Marburg
10.45 – 11.10 Coffee Break
Trafficking and transport (Chair: Dan Mulvihill / Zeynep Ökten)
11.10 – 11.40 Actin assembly in cellular stress response pathways
Kenneth Campellone, University of Connecticut
11.40 – 12.10 How to navigate through the cytoskeletal maze
Zeynep Ökten, TU Munich
12.10 – 12.40 Selected talks from abstracts
[T18] VASP boosts protrusive activity of macroendocytic cups and drives phagosome rocketing after internalization
Sarah Körber (YIA) MHH
[T19] Elongator is a microtubule associated protein that controls central spindle asymmetry and polarized trafficking of cell fate determinants
Vicente Planelles-Herrero (YIA) LMB Cambridge
12.40 – 14.00 Lunch
Mechanosignalling (Chair: Klemens Rottner/ Ewa K. Paluch)
14.00 – 14.30 Exploring cell adhesion on molecular scales
Carsten Grashoff, WWU Münster
14.30 – 15.00 Assembly of actomyosin stress fibers in migrating cells
Pekka Lappalainen, University of Helsinki
15.00 – 16:00 Selected talks from abstracts
[T20] Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration
Gregory Giannone, University Bordeaux
[T21] Vinculin regulates lamellipodium actin and protrusion dynamics
Ingo Thievessen, Friedrich-Alexander-University of Erlangen-Nuremberg
[T22] Role of contractility in the chiral swirling of endothelial cells
Ghina Badih, Université Grenoble Alpes
[T23] Trapping non-muscle myosin II into filament assembly and amplification
Melissa Quintanilla, Loyola University Chicago
16.00 – 16.30 Coffee Break
16.30 – 19.15 Poster session II: P34 - P64
19.30 – 21.30 Dinner @ Altes Rathaus
Thursday 19 May 2022
Structural Biology of the Cytoskeleton (Chair: Roberto Dominguez/ Dietmar Manstein)
09.00 – 10:00 Selected talks from abstracts
[T5] ArpC5 Isoform Specificity Affects Arp2/3 Complex Branch Junction Structure, Lamellipodium Architecture and Cell Motility
Florian Fäßler (YIA), ISTA, Klosterneuburg
[T6] Molecular-scale visualization of sarcomere contraction within native cardiomyocytes
Jonathan Schneider, MPI Martinsried
[T7] Structural basis of actin filament assembly and aging
Wout Oosterheert (YIA), MPI Dortmund
[T8] Resolving actin polarity dynamics during cell spreading
Wen-Lu Chung (YIA), University of Zurich
Regulation of Adhesion (Chair: Theresia Stradal / Pekka Lappalainen)
10.00 – 10:30 Mechanosensing via E-cadherin
Alpha S. Yap, University of Queensland
10.30 – 11.00 Coffee Break
11.00 – 11.30 How tension directs tensin-mediated fibronectin fibrillogenesis
Christoph Ballestrem, University of Manchester
11.30 – 12.00 Selected talks from abstracts
[T24] Dual function of Swip1/EFHD2 controlling integrin endocytosis and vesicle movement in breast cancer cells
Paulina Moreno Layseca (YIA), University of Turku
[T25] Adhesion site turnover in motile malaria parasites is modulated by Coronin
Julia Sattler, Universität Heidelberg
12.00 – 13.30 Lunch
13.30 – 14.00 Announcement of Awards and Poster Prizes
Emerging imaging techniques (Chair: Michelle Peckham / Georgios Tsiavaliaris)
14.0 – 14.30 Revealing the mechanism of actin polymerisation with mass photometry
Philipp Kukura, University of Oxford
14.30 – 15.00 Actin-induced compartmentalization of the cellular plasma membrane -
an advanced super-resolved optical microscopy study
Christian Eggeling, Universität Jena
15.10 – 15.55 Selected talks from abstracts
[T26] EN-PAINT allows live cell imaging of intracellular structures with sub-diffraction resolution
Simon Hennig, MHH
[T27] IntAct: a non-disruptive internal tagging strategy to study actin isoform organization and function
Koen van den Dries, Radboud Institute for Molecular Life Sciences
[T28] Thermophoretic Biosensors: Novel Tools in Cytoskeletal Research
Peter Franz, MHH
15.45 – 16.00 Concluding remarks
16:00 End of meeting